Anti-EZH1 Polyclonal Antibody for WB and ELISA - Q92800

Core Product Specifications and Parameters

Biological Background: EZH1 Function and Localization

• EZH1 (Enhancer of zeste homolog 1, also known as ENX-2 or KMT6B) is a polycomb group protein and the catalytic subunit of the PRC2/EED-EZH1 complex.
• It functions as a histone-lysine N-methyltransferase that specifically mono-, di-, and trimethylates 'Lys-27' of histone H3 (H3K27me1, H3K27me2, H3K27me3), leading to transcriptional repression of target genes.
• Compared to its paralog EZH2, EZH1 is less abundant in embryonic stem cells and possesses weaker methyltransferase activity, yet it is essential for embryonic stem cell derivation and self-renewal, indicating a role in maintaining stem cell identity.
• EZH1 localizes to the nucleus, consistent with its function in chromatin modification and gene silencing.
• The protein undergoes alternative splicing and can be modified by ubiquitin-like conjugation, as reflected by UniProt keywords.
• As a SET domain-containing methyltransferase, EZH1 uses S-adenosyl-L-methionine as a cofactor and is classified as a transferase.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 160–280 of human EZH1; sequence conservation across species supports the observed cross-reactivity with mouse and rat.
• For Western blot analysis, a specific band is expected near 85 kDa; using pre-stained markers and appropriate loading controls is recommended to verify specificity.
• In ELISA, the recombinant immunogen can serve as a positive control, but assay conditions should be optimized for each experimental setup.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to validate performance in the relevant sample system before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop partner for IVD raw materials, offering researchers and diagnostic manufacturers reliable access to antibodies like this EZH1 polyclonal antibody for epigenetic and chromatin research. Our global supply chain supports validated antibody pairs, optimized monoclonal and polyclonal formats, and bulk ancillary reagents to streamline assay development from concept to clinic. For rare targets or customized sourcing needs, CamelBio’s extensive network ensures efficient procurement of specialty raw materials, helping you bridge innovation and clinical application.