Anti-EYA3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q99504

Core Product Specifications and Parameters

Biological Background: EYA3 Function and Localization

• Protein phosphatase EYA3, also known as Eyes absent homolog 3, is encoded by the EYA3 gene (UniProt: Q99504). Alternative splicing generates multiple isoforms.
• Core function: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX. This modification plays a central role in distinguishing DNA repair vs. apoptotic responses to genotoxic stress.
• Promotes efficient DNA repair by dephosphorylating H2AX, thereby recruiting DNA repair complexes containing MDC1. Related references: PMID:19234442 PMID:19351884
• Functions as a transcription coactivator for SIX family proteins (SIX1, SIX2, SIX4, SIX5), linking phosphatase activity to developmental gene regulation.
• Subcellular localization: Found in both cytoplasm and nucleus, consistent with dual roles in signaling and chromatin regulation.
• Classified under multiple functional categories: Chromatin regulator, DNA damage, DNA repair, developmental protein, transcription, and protein phosphatase.
• May be involved in eye development (based on sequence homology and functional studies).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-300 of human EYA3, covering the N-terminal region. This sequence is highly conserved, but users should verify reactivity in specific species or splice variants.
• For Western blot, the predicted protein weight is ~63 kDa (theoretical MW 62.7 kDa); observe a band near this molecular weight in lysates from human and rat samples.
• Based on the provided applications (WB, IF/ICC, ELISA), consider starting with typical antibody dilutions and optimizing for the specific sample and detection system.
• For immunofluorescence/immunocytochemistry, note the cytoplasmic and nuclear localization; appropriate fixation and permeabilization are critical for signal detection.
• Validate specificity using appropriate controls (e.g., siRNA knockdown, peptide blocking) to confirm target recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD manufacturers and research laboratories, offering a streamlined supply chain for high-quality raw materials like this EYA3 polyclonal antibody. Beyond standard antibody products, we provide validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Our expertise in DNA repair, transcriptional regulation, and developmental biology markers supports your assay development from concept to clinic.