Anti-EYA1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q99502

Core Product Specifications and Parameters

Biological Background: EYA1 Function and Localization

• EYA1 (Eyes absent homolog 1) is a protein phosphatase and transcriptional coactivator for SIX family members (SIX1, SIX2, SIX4, SIX5), playing critical roles in development.
• It dephosphorylates Tyr-142 of histone H2AX, promoting DNA repair by recruiting MDC1-containing complexes, thereby distinguishing repair from apoptosis. Related references: PMID:19234442
• As a transcriptional coactivator, EYA1 regulates gene expression during organogenesis, contributing to craniofacial, skeletal, kidney, and ear development.
• Subcellular localization includes both cytoplasm and nucleus, consistent with its dual roles in signaling and transcription.
• In the embryo, EYA1 is highly expressed in kidney and weakly in brain and lung; in adults, high expression in heart and skeletal muscle, weak in brain and liver.
• Keywords annotate EYA1 involvement in DNA repair, chromatin regulation, transcription, developmental processes, and disease variants associated with deafness and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 200-300 of human EYA1; this region may recognize multiple isoforms and facilitate detection in various applications.
• For WB, consider testing a range of antibody dilutions and validate band specificity using appropriate controls, as EYA1 has a predicted molecular weight of ~65 kDa.
• In IHC-P, optimize antigen retrieval and antibody concentration to achieve specific staining, and include tissue controls with known EYA1 expression.
• For ELISA, determine the optimal coating and detection conditions, and validate with recombinant protein or cell lysates as appropriate.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable partner for sourcing high-quality IVD raw materials, offering a broad portfolio of validated antibodies, antigens, and reagents. This anti-EYA1 polyclonal antibody exemplifies our commitment to supporting developmental biology and DNA repair research with rigorously tested products. In addition to single antibodies, we provide matched antibody pairs, bulk reagents, and customized sourcing for rare targets to streamline your assay development from concept to clinic.