Anti-EXT2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q93063

Core Product Specifications and Parameters

Biological Background: EXT2 Function and Localization

• Encodes exostosin-2, a type II transmembrane glycosyltransferase that serves as a critical subunit of the heparan sulfate (HS) co-polymerase; alternative names include Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase and Multiple exostoses protein 2.
• Forms a heterodimeric complex with EXT1 to constitute the active HS polymerase; together they catalyze the processive elongation of the HS backbone by alternating addition of D-glucuronic acid (GlcA) and N-acetyl-D-glucosamine (GlcNAc) residues from their respective UDP-sugar donors. Related references: PMID:22660413, PMID:36402845, PMID:36593275
• The EXT1 subunit provides the glucuronosyltransferase activity, while EXT2 is responsible for the N-acetylglucosaminyltransferase activity; both are required for full polymerase function and HS chain synthesis. Related references: PMID:36402845, PMID:36593275
• The resulting heparan sulfate proteoglycans are ubiquitous components of the extracellular matrix and cell surfaces, playing essential roles in tissue homeostasis, growth factor signaling, and cell adhesion. Related references: PMID:19344451, PMID:22660413
• Primarily localizes to the Golgi apparatus membrane, with presence in the cis-Golgi network and endoplasmic reticulum membrane; a secreted form has also been reported.
• Widely expressed across tissues.
• Mutations in EXT2 are associated with hereditary multiple exostoses, an autosomal dominant disorder characterized by cartilage-capped bony outgrowths; the protein is also classified as a tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 80–340) encompasses the catalytic domain of EXT2; the resulting polyclonal antibody is expected to recognize multiple epitopes within this region, providing robust detection for denaturing Western blotting and native applications like IF/ICC.
• For Western blotting, consider a primary antibody dilution range of 1:500–1:2000 and validate against the predicted molecular weight of ~82 kDa; inclusion of appropriate controls (e.g., EXT2-overexpressing lysates or siRNA-treated samples) is recommended.
• In immunofluorescence/ICC, EXT2 typically displays a perinuclear Golgi-like staining pattern; membrane permeabilization with 0.1% Triton X-100 can improve accessibility while preserving organelle morphology.
• ELISA performance is supported by the recombinant protein immunogen; consider using the antibody as a capture or detection reagent in sandwich ELISA setups for HS-related biomarker research.
• Cross-reactivity with mouse and rat EXT2 has been demonstrated, enabling use in rodent models; however, it is advisable to verify specificity in the relevant species and tissue before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies procurement for diagnostic manufacturers, research laboratories, and academic institutions by providing a centralized channel for high-quality IVD raw materials. This EXT2 antibody supports in-depth investigation into heparan sulfate biology and hereditary multiple exostoses, and CamelBio offers a comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing capabilities. With a focus on bridging concept-to-clinic workflows, CamelBio ensures reliable access to critical reagents for your diagnostic and translational research needs.