Anti-EXT1 Rabbit Polyclonal Antibody for WB, ELISA - Q16394

Core Product Specifications and Parameters

Biological Background: EXT1 Function and Localization

• EXT1 (Exostosin-1) is a glycosyltransferase that forms the heterodimeric heparan sulfate polymerase complex with EXT2, essential for heparan sulfate chain elongation.
• The enzyme catalyzes the alternating transfer of glucuronic acid and N-acetylglucosamine residues, with EXT1 providing the glucuronosyltransferase activity. Related references: PMID:36402845, PMID:36593275
• Heparan sulfate proteoglycans, synthesized by EXT1-containing complexes, are ubiquitous extracellular matrix components involved in tissue homeostasis and signaling. Related references: PMID:10639137, PMID:11391482, PMID:22660413
• EXT1 is a membrane-anchored protein with a signal-anchor transmembrane domain, predominantly localized to the Golgi apparatus (cis-Golgi network) and endoplasmic reticulum membranes.
• Widely expressed across tissues, EXT1 mutations cause hereditary multiple exostoses; the protein is also recognized as a tumor suppressor.
• Classified under keywords: Glycosyltransferase, Golgi apparatus, Membrane, Tumor suppressor, Disease variant, Metal-binding (manganese).

Experimental Guidance and Technical Tips

• The immunogen is an internal region (aa 300–400) of human EXT1; the polyclonal antibody likely recognizes multiple epitopes, suitable for detecting both native and denatured protein in WB.
• For Western blot, consider using reducing conditions and a loading control; the expected molecular weight is approximately 86 kDa.
• In ELISA applications, the immunizing peptide can serve as a positive control to confirm antibody specificity and assay sensitivity.
• Cross-reactivity with rat has been reported by the supplier; if testing other species, antibody reactivity should be validated in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and researchers to provide high-quality raw materials, including validated antibody pairs and polyclonal antibodies like this EXT1-targeted reagent. Whether you need bulk reagents, customized antibody production, or ancillary materials, our team supports your project from early R&D to clinical application, ensuring a seamless supply chain for heparan sulfate biology and related disease research.