Anti-Exportin-6 (XPO6) Rabbit Polyclonal Antibody for WB and ELISA - Q96QU8

Core Product Specifications and Parameters

Biological Background: Exportin-6 Function and Localization

• Exportin-6 (XPO6), also known as Ran-binding protein 20 (RANBP20), is a member of the importin-beta superfamily of nuclear transport receptors and exhibits alternative splicing.
• It mediates the Ran-dependent nuclear export of actin and profilin-actin complexes in somatic cells, playing a critical role in cytoskeletal regulation.
• XPO6 belongs to the exportin family and operates through a classical nuclear export pathway involving recognition of cargoes with nuclear export signals.
• Subcellularly, Exportin-6 shuttles between the nucleus and cytoplasm, consistent with its transport function.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or localization.
• The full-length protein has a predicted molecular weight of approximately 129 kDa, matching the observed product size.

Experimental Guidance and Technical Tips

• The recombinant immunogen spans a central region of XPO6, which may help in detecting both full-length and potential isoforms.
• For Western blotting, a band at approximately 129 kDa is expected; consider using whole-cell lysates from human or mouse cell lines based on confirmed cross-reactivity.
• In ELISA, the antibody can be used for detection of XPO6 protein; consider testing direct or sandwich formats with appropriate standards.
• Validate antibody specificity using positive and negative controls (e.g., lysates from cells with known XPO6 expression or knockdown/knockout models).
• Cross-reactivity with mouse XPO6 enables cross-species studies; always validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting nuclear transport and cytoskeleton research with high-quality reagents like this XPO6 Rabbit pAb. We provide validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets, bridging your concept to clinic with reliable supply chains.