Anti-Exportin 5 (XPO5) Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q9HAV4

Core Product Specifications and Parameters

Biological Background: Exportin 5 Function and Localization

• Exportin-5 (XPO5), also known as Ran-binding protein 21, is a ~136 kDa nuclear transport receptor member of the importin-β superfamily.
• It specifically mediates the nuclear export of double-stranded RNA (dsRNA) and micro-RNA precursors (pre-miRNAs) in a RanGTP-dependent manner (PubMed:14631048, PubMed:14681208, PubMed:15613540). Related references: PMID:14631048 PMID:14681208 PMID:15613540
• XPO5 recognizes dsRNAs with a minimum length of 15 base pairs, a short 3′-overhang, and no 5′-overhang, a structural basis for selective cargo export (PubMed:19965479). Related references: PMID:19965479
• In addition to pre-miRNAs, XPO5 exports viral dsRNAs such as adenovirus VA1, linking it to host-virus interaction and innate immunity.
• The export cycle involves cooperative binding of XPO5 to Ran-GTP and cargo in the nucleus, docking to nucleoporins, and cytoplasmic release upon GTP hydrolysis.
• XPO5 is predominantly localized in the nucleus but shuttles to the cytoplasm, with expression detected in heart, brain, placenta, lung, skeletal muscle, kidney, and pancreas.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity or protein interactions.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 925-1204) of human Exportin 5, which is highly conserved across human, mouse, and rat, supporting cross-reactivity.
• For Western blot, the predicted molecular weight is 136 kDa; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/ICC, expect predominantly nuclear staining with some cytoplasmic localization.
• For IP, use standard protocols with careful optimization of antibody concentration.
• This polyclonal antibody may recognize multiple isoforms or degradation products; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw materials, offering validated antibodies and ancillary reagents for accelerated diagnostic development. This Exportin 5 (XPO5) polyclonal antibody supports research into RNA transport, gene silencing mechanisms, and viral-host interactions. Our supply capabilities include bulk antibody sourcing, custom antibody pairs, and technical consulting from concept to clinic.