Anti-Exportin 2 (XPO2) Rabbit Monoclonal Antibody for WB and ELISA - P55060

Core Product Specifications and Parameters

Biological Background: Exportin-2 Function and Localization

• Exportin-2, also known as CAS, CSE1, XPO2, or importin-alpha re-exporter, is encoded by the CSE1L gene.
• Functions as the export receptor for importin-alpha, mediating its re-export from the nucleus to the cytoplasm after cargo release.
• In the nucleus, forms a cooperative trimeric complex with importin-alpha and GTP-bound Ran, docking to nucleoporins for transport through the nuclear pore complex.
• Upon cytoplasmic disassembly, Ran-GTP hydrolysis stimulated by RANBP1 and RANGAP1 releases importin-alpha, allowing Exportin-2 to recycle back to the nucleus.
• The asymmetric distribution of Ran-GTP and Ran-GDP between nucleus and cytoplasm confers directionality to nuclear export.
• Detected in brain, placenta, ovary, testis, and trachea, with high expression in testis and proliferating cells.
  Related references:
  PMID:10331944
  PMID:7479798
• Localizes to both the cytoplasm and nucleus.
• Post-translationally modified by acetylation and phosphorylation; subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen targets a C-terminal peptide (aa 872–971) of Exportin 2; for Western blot, use a 110 kDa protein ladder and consider extended transfer times for high-molecular-weight proteins.
• This rabbit monoclonal antibody is suitable for ELISA; for sandwich ELISA development, evaluate pairing with a capture antibody targeting a non-overlapping Exportin 2 epitope.
• The antibody recognizes both human and mouse Exportin 2; validate reactivity in the appropriate cell or tissue lysates to confirm specificity and expected band size.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting for research and diagnostic applications. This anti-Exportin 2 antibody serves as a key reagent for studying nucleocytoplasmic transport pathways; we support your workflow with validated monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare target proteins. Reach out to leverage our integrated supply chain and accelerate your assay development.