Anti-EXOSC3 Rabbit Polyclonal Antibody for WB, ELISA - Q9NQT5

Core Product Specifications and Parameters

Biological Background: EXOSC3 Function and Localization

• EXOSC3, also known as exosome complex component RRP40, ribosomal RNA-processing protein 40, or p10, is a core component of the RNA exosome, a multi-subunit complex with 3'→5' exoribonuclease activity.
• As part of the catalytic inactive Exo-9 core, EXOSC3 stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5, serving as a scaffold for the association of catalytic subunits and accessory factors.
• The RNA exosome participates in nuclear processing and maturation of stable RNAs, including rRNA, snRNA, and snoRNA, and removes processing by-products and pervasive non-coding transcripts such as antisense RNA and PROMPTs.
• In the cytoplasm, the complex is involved in general mRNA turnover, specifically degrading inherently unstable mRNAs containing AU-rich elements (AREs) in their 3' UTRs, and contributes to RNA surveillance by preventing translation of aberrant mRNAs.
• EXOSC3-containing exosome plays roles in specialized processes: degradation of histone mRNA, Ig class switch recombination (CSR) and somatic hypermutation (SHM) by targeting AICDA deamination activity to dsDNA substrates.
• Subcellularly, EXOSC3 localizes predominantly to the nucleolus, but also to the nucleoplasm and cytoplasm, reflecting its diverse functions in RNA metabolism.
• Post-translational modifications include acetylation and ubiquitin conjugation (isopeptide bond), and the protein is associated with disease variants linked to neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-275) of human EXOSC3, which is conserved across species; this antibody is therefore expected to recognize the full-length protein in human, mouse, and rat samples.
• For Western blotting, a band around 30 kDa is anticipated based on the calculated molecular weight; use appropriate positive controls (e.g., HeLa, HEK293, or mouse/rat tissue lysates) and validate with siRNA knockdown or knockout controls if possible.
• In ELISA, consider coating with recombinant EXOSC3 protein or cell lysates, and optimize antibody dilution and detection system empirically.
• Due to the polyclonal nature, lot-to-lot consistency should be confirmed for critical applications; we recommend performing in-house validation in your specific assay context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to deliver validated IVD raw materials, including high-performance antibodies like this EXOSC3 Rabbit pAb for advanced RNA biology studies. Our portfolio spans validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, streamlining your transition from concept to clinic. Whether you require rare target sourcing or one-stop procurement support, CamelBio is your trusted supply partner.