Anti-EXOSC1 Polyclonal Antibody for WB and ELISA - Q9Y3B2

Core Product Specifications and Parameters

Biological Background: EXOSC1 (CSL4) Function and Localization

• EXOSC1, also known as CSL4, is a non-catalytic peripheral component of the RNA exosome complex, a multi-subunit 3'→5' exoribonuclease essential for RNA processing and degradation.
• The protein stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC6 and EXOSC8, forming the catalytically inactive Exo-9 core complex that presents RNA for ribonucleolysis.
• In the nucleus, the exosome complex participates in maturation of stable RNAs (rRNA, snRNA, snoRNA), removal of processing by-products and pervasive transcripts (antisense RNA, PROMPTs), and quality control of defective mRNAs. It may also facilitate immunoglobulin class switch recombination and somatic hypermutation by targeting AICDA activity.
• In the cytoplasm, EXOSC1-containing exosome complexes mediate general mRNA turnover, degradation of ARE-containing unstable mRNAs, and surveillance pathways that prevent translation of aberrant transcripts, including histone mRNA decay.
• Subcellularly, EXOSC1 localizes to the nucleus, nucleolus, and cytoplasm, consistent with its roles in both nuclear and cytoplasmic RNA metabolism.
• The protein is subject to phosphorylation (as indicated by proteomics), and mutations in EXOSC1 are associated with neurodegenerative diseases, linking its RNA processing functions to neuronal health.
• Additional annotations include RNA-binding and rRNA processing activities, reflecting its direct interaction with RNA substrates within the exosome scaffold.

Experimental Guidance and Technical Tips

• For Western blot, consider titrating the antibody (e.g., 1:500–1:2000) to detect endogenous EXOSC1 in human cell lysates, given its predicted molecular weight of ~21 kDa.
• The immunogen covers the N-terminal 195 amino acids of human EXOSC1, which may allow recognition of multiple isoforms or degradation products; verify specificity using positive and negative controls.
• In ELISA, this antibody can be used for direct detection or as a capture/detection reagent; confirm linearity and sensitivity with recombinant protein standards.
• Since the antibody is a polyclonal, it may offer robust signal but batch-to-batch variability can occur; include appropriate controls and re-validate when switching lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For RNA biology and exosome complex studies, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Connect with us to streamline your procurement of high-quality EXOSC1 antibodies and related products for your diagnostic or research applications.