Anti-EXOC5 Rabbit Polyclonal Antibody for WB and ELISA - O00471

Core Product Specifications and Parameters

Biological Background: EXOC5 Function and Localization

• EXOC5, also known as Sec10, is a core subunit of the exocyst complex, an evolutionarily conserved octameric protein assembly.
• The exocyst complex mediates the tethering and docking of post-Golgi secretory vesicles to specific sites on the plasma membrane prior to SNARE-mediated fusion.
• EXOC5 localizes primarily to the cytoplasm and accumulates at the midbody during cytokinesis.
• The protein contains coiled-coil domains, which are likely involved in protein-protein interactions within the exocyst complex.
• EXOC5 is subject to post-translational modifications, including acetylation and phosphorylation, which may regulate its function or complex assembly.
• It is ubiquitously expressed across human tissues, consistent with its role in fundamental secretory processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 340-610 of EXOC5, a region containing predicted coiled-coil motifs; this fragment likely yields antibodies that recognize the native protein.
• For Western blot, a signal near 82 kDa is expected; validate in relevant human, mouse, or rat cell lysates.
• For ELISA, consider titrating the antibody to determine optimal working concentration for your specific assay format.
• Cross-reactivity with additional species may be tested if needed for your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering validated antibodies and bulk reagents for targets including exocyst complex components like EXOC5. From optimized polyclonal antibodies to ancillary reagents and rare-target sourcing, our supply chain solutions support your research and diagnostic development.