Anti-EXOC4 Polyclonal Antibody for WB and ELISA - Q96A65

Core Product Specifications and Parameters

Biological Background: EXOC4 Function and Localization

• EXOC4, also known as Sec8, is a core component of the exocyst complex, an evolutionarily conserved octameric protein assembly essential for exocytosis.
• It mediates the tethering and docking of post-Golgi secretory vesicles to specific fusion sites on the plasma membrane, enabling polarized secretion and cell surface expansion.
• The exocyst complex participates in diverse cellular processes, including cell growth, cytokinesis, primary ciliogenesis, and neuronal morphogenesis.
• EXOC4 localizes to the midbody and midbody ring during cytokinesis, and is also found at the centrosome and in cell projections.
• Post-translational modifications such as acetylation and phosphorylation may regulate EXOC4 stability, localization, or interaction with other exocyst subunits.
• Alternative splicing generates multiple EXOC4 isoforms, which could contribute to tissue-specific or context-dependent functions.
• Structural analyses reveal that EXOC4 contains long coiled-coil domains that are critical for exocyst complex assembly and interaction with vesicular and plasma membrane components.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal 1‑280 amino acids of human EXOC4; consider that the antibody may recognize this region in denaturing or native conditions.
• For Western blotting, validate the antibody in lysates from human, mouse, or rat cells, and confirm the band near the predicted molecular weight of 110 kDa.
• In ELISA applications, optimize coating and blocking conditions, and test the antibody in both direct and sandwich formats as needed.
• Since the exocyst complex is evolutionarily conserved, cross-reactivity with other species should be verified experimentally in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined supply platform for diagnostic manufacturers and researchers, providing validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents for exocyst and vesicular trafficking studies. We specialize in sourcing rare‑target raw materials and can support bulk procurement of this EXOC4 antibody alongside complementary exocyst complex components. Let CamelBio be your bridge from concept to clinic with reliable, one‑stop access to IVD raw materials and technical resources.