Anti-EXOC3 Polyclonal Antibody for WB, ELISA - O60645

Core Product Specifications and Parameters

Biological Background: EXOC3 Function and Localization

• EXOC3 (Exocyst complex component 3), also known as Sec6, is a core subunit of the evolutionarily conserved exocyst complex.
• The exocyst complex mediates the tethering and spatial targeting of post-Golgi secretory vesicles to specific sites on the plasma membrane, facilitating SNARE-mediated fusion and polarized secretion.
• EXOC3 localizes to the cytoplasm and perinuclear region, and is dynamically recruited to the Golgi apparatus, midbody, and cell projections such as growth cones and neuron projections.
• Acetylation modifications of EXOC3 have been identified through proteomic approaches, indicating potential post-translational regulatory mechanisms.
• At the protein level, EXOC3 is expressed in the epididymis, though the exocyst complex functions ubiquitously in exocytic processes across various tissues.
• Keywords associated with EXOC3 include acetylation, cell projection, cytoplasm, exocytosis, Golgi apparatus, and protein transport, underscoring its role in cellular secretion pathways.

Experimental Guidance and Technical Tips

• For Western blotting, the expected band is approximately 86 kDa (mature protein predicted at ~85.6 kDa). Use a suitable gel percentage (e.g., 8–10% SDS-PAGE) and optimize transfer conditions for high molecular weight proteins.
• In ELISA, consider checkerboard titrations to determine optimal antibody and antigen concentrations; include appropriate positive and negative controls for human samples.
• The immunogen spans amino acids 396–745, covering the C-terminal half of full-length EXOC3. This design may enable detection of both full-length and potential cleavage products; validate specificity in your experimental system.
• Confirm antibody reactivity in human cell lines or tissues known to express EXOC3, and be aware that cross-reactivity with other species has not been tested, so validation is required for non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with one-stop access to high-quality IVD raw materials, including this anti-EXOC3 polyclonal antibody. We support exocytosis and cell biology research through validated antibody pairs, bulk ancillary reagents, and sourcing of rare target raw materials. Contact us to streamline your procurement workflow from discovery to clinical application.