Anti-EXOC3 Monoclonal Antibody for WB, ELISA - O60645

Core Product Specifications and Parameters

Biological Background: EXOC3 Function and Localization

• EXOC3, also known as Sec6, is a core subunit of the exocyst complex, an octameric protein assembly that tethers secretory vesicles to the plasma membrane prior to SNARE-mediated fusion.
• The exocyst complex, including EXOC3, plays a critical role in polarized exocytosis by directing vesicle docking at specific sites, essential for processes such as cell growth, migration, and neurotransmitter release.
• EXOC3 localizes primarily to the cytoplasm, with enrichment in the perinuclear region, and is also found at the growth cone of developing neurons, the midbody during cytokinesis, and the Golgi apparatus.
• It is acetylated, suggesting regulatory post-translational modification that may influence complex stability or localization.
• EXOC3 is expressed in the epididymis at the protein level, with broader detection at the mRNA level, indicating potential roles in both systemic and reproductive secretory pathways.

Experimental Guidance and Technical Tips

• For western blot (WB), consider using reduced and denatured sample conditions to expose the immunogen region (aa 650-745), which may improve antibody binding.
• In ELISA, the recombinant immunogen fragment may serve as a positive control to assess antibody specificity and sensitivity; native protein recognition should be validated in appropriate lysates.
• Given cross-reactivity with human, mouse, and rat, the antibody can be evaluated across species, but conditions may require optimization for each.
• The protein is expressed in epididymis and has a predicted molecular weight of ~86 kDa; thus, tissue or cell lines with high secretory activity are ideal for functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering validated antibodies, including this anti-EXOC3 monoclonal antibody, to support research in exocytosis, vesicle trafficking, and secretion pathways. We provide comprehensive sourcing solutions—from optimized antibody pairs and bulk reagents to rare target raw materials—ensuring seamless scale-up from discovery to diagnostic development.