Anti-EXOC2 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q96KP1

Core Product Specifications and Parameters

Biological Background: EXOC2 Function and Localization

• EXOC2, also known as Sec5, is a subunit of the exocyst complex, a conserved octameric tethering complex that mediates the initial contact between secretory vesicles and the plasma membrane.
• It plays a critical role in exocytosis by directing post-Golgi vesicles to specific docking and fusion sites, essential for polarized secretion and cell surface expansion.
• The exocyst complex is involved in various cellular processes including cytokinesis, ciliogenesis, and cell migration.
• EXOC2 localizes to the midbody and midbody ring during cell division, indicating a role in the final stages of cytokinesis.
• Widely expressed across tissues, EXOC2 shows highest expression in brain and placenta, suggesting specialized functions in neural and placental development.
• Post-translational modifications include acetylation and phosphorylation; the protein is associated with coiled-coil domains that likely mediate protein-protein interactions within the exocyst complex.
• Mutations or dysregulation of EXOC2 have been linked to intellectual disability and neurodegeneration, highlighting its importance in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen was designed using the N-terminal region (amino acids 1-220) of human EXOC2; this region may offer good specificity and limited cross-reactivity with other exocyst subunits.
• For Western blot analysis, a band of approximately 104 kDa is expected; consider using 6-8% SDS-PAGE gels for optimal resolution of high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), appropriate fixation/permeabilization protocols should be empirically determined to detect EXOC2 at the midbody or plasma membrane.
• The antibody is predicted to cross-react with mouse and rat EXOC2 based on sequence similarity; validation in the relevant species and tissue is recommended.
• For immunoprecipitation (IP) and ELISA, antibody titration and optimization of buffer conditions are advised to achieve the best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials. This anti-EXOC2 antibody supports studies in intracellular trafficking, exocytosis, and neurobiology. Beyond antibodies, our supply chain covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your development from concept to clinic.