Anti-EXOC2 Rabbit Monoclonal Antibody for WB, ELISA - Q96KP1

Core Product Specifications and Parameters

Biological Background: EXOC2 Function and Localization

• EXOC2 (also known as Sec5, SEC5L1) is a core component of the exocyst complex, an evolutionarily conserved octameric protein assembly that tethers secretory vesicles to the plasma membrane prior to SNARE-mediated fusion.
• The exocyst complex plays a critical role in polarized exocytosis, directing vesicle trafficking to specific membrane domains, which is essential for processes such as cell growth, division, and migration.
• Within the complex, EXOC2/Sec5 directly binds to several other subunits and is thought to help orchestrate the spatial and temporal assembly of the exocyst at active fusion sites.
• EXOC2 localizes prominently to the midbody and midbody ring during cytokinesis, where it facilitates abscission by targeting recycling endosomes to the intercellular bridge.
• Expression is ubiquitous but highest in brain and placenta, reflecting its importance in highly secretory cell types and in neural development.
• Post-translational modifications of EXOC2 include acetylation and phosphorylation, which may regulate its activity, localization, or interactions with other exocyst subunits or trafficking regulators.
• Mutations in EXOC2 have been linked to human diseases, including neurodevelopmental disorders with intellectual disability and neurodegeneration, underscoring its essential role in neuronal function.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–100), which is well conserved across human, mouse, and rat, likely accounting for the observed cross-reactivity.
• For Western blot, the predicted molecular weight is 104 kDa; consider using a 7.5% or 8% SDS-PAGE gel for optimal resolution.
• For ELISA, titrate the antibody to determine the optimal working concentration in your specific assay format.
• Validate the antibody in your target tissue or cell line, as EXOC2 expression levels vary (e.g., highest in brain and placenta).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with reliable access to high-quality IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-EXOC2 clone. Whether you need bulk ancillary reagents, custom antibody development, or assistance sourcing rare targets, our one-stop procurement platform supports your entire workflow from concept to clinic.