Anti-EVA1A Polyclonal Antibody for WB, IHC-P, ELISA - Q9H8M9

Core Product Specifications and Parameters

Biological Background: EVA1A Function and Localization

• Protein eva-1 homolog A (EVA1A), also known as FAM176A or TMEM166, is a transmembrane protein that acts as a regulator of programmed cell death.
• EVA1A mediates both autophagy and apoptosis, linking these two distinct cell death pathways.
• It localizes to the endoplasmic reticulum membrane and lysosome membrane, implicating it in organelle-associated cell death processes.
• Tissue expression is observed in lung, kidney, liver, pancreas, and placenta, but not in heart and skeletal muscle.
• The protein is classified as a phosphoprotein and features a transmembrane helix, consistent with its membrane localization.
• UniProt keywords include apoptosis, autophagy, endoplasmic reticulum, lysosome, membrane, and reference proteome, highlighting its role in cell death and membrane biology.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 90-152 of human EVA1A, a region likely exposed on the cytoplasmic side of the membrane; consider this when designing detection protocols.
• For Western blotting, validate the antibody using lysates from human, mouse, or rat cell lines expressing EVA1A; include negative controls from tissues with low expression like heart or skeletal muscle.
• In immunohistochemistry (IHC-P), perform antigen retrieval optimization and include appropriate isotype controls to assess staining specificity.
• ELISA applications may benefit from checkerboard titrations to determine optimal coating antigen and antibody concentrations.
• Cross-reactivity with mouse and rat allows comparative studies; however, always verify in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For apoptosis and autophagy research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Let us be your bridge to reliable supply for EVA1A and related cell death targets.