Anti-ETV7 Polyclonal Antibody for WB, ELISA - Q9Y603

Core Product Specifications and Parameters

Biological Background: ETV7 Function and Localization

• ETV7 (ETS translocation variant 7) is a transcription factor of the ETS family, also named TEL2, TELB, or TEL-2. It plays a role in gene regulation through DNA binding.
• It acts as a transcriptional repressor by specifically recognizing the DNA sequence 5'-CCGGAAGT-3', thereby modulating target gene expression.
• Alternative splicing produces multiple isoforms; isoform A and isoform C may lack canonical repressor activity, suggesting isoform-specific functional diversity.
• ETV7 localizes to the nucleus, consistent with its function as a DNA-binding protein involved in transcription regulation.
• Expression is enriched in hematopoietic tissues, indicating a role in blood cell development and differentiation.
• The protein is subject to proteomics identification and is annotated with keywords including alternative splicing, DNA-binding, nucleus, repressor, and transcription regulation, reflecting its regulatory nature.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–210) of human ETV7, which may detect multiple isoforms; nonetheless, isoform specificity should be confirmed in relevant models.
• For Western blotting, the target protein has a predicted mass of approximately 39 kDa (UniProt: 38998 Da). Use lysates from hematopoietic cell lines as positive controls and validate band size.
• When performing ELISA, consider optimizing coating conditions and antibody titers. For sandwich ELISA, pairing with a suitable detection antibody is advisable.
• Due to potential low endogenous expression, enrichment of nuclear extracts or use of high-sensitivity detection reagents may improve signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies IVD manufacturers and research laboratories with a comprehensive portfolio of antibodies, recombinant proteins, and ancillary reagents. For transcription factor targets like ETV7, we offer validated antibody pairs, bulk raw materials, and procurement support to streamline assay development. Our one-stop platform bridges sourcing gaps, from concept to clinical application.