Anti-ETV6 Rabbit Polyclonal Antibody for WB, ELISA - P41212

Core Product Specifications and Parameters

Biological Background: ETV6 Function and Localization

• ETV6 (ETS translocation variant 6), also known as TEL or TEL1, is a member of the ETS family of transcription factors.
• Functions as a transcriptional repressor by binding to the specific DNA sequence 5'-CCGGAAGT-3'.
• Plays a critical role in normal hematopoiesis and is frequently involved in chromosomal rearrangements associated with hematological malignancies.
• Localized predominantly in the nucleus.
• Expressed ubiquitously across various tissues.
• Post-translational modifications include acetylation and phosphorylation, and it can be modified by ubiquitin-like conjugation.
• The protein is a known proto-oncogene with DNA-binding and repressor activities, and it is a reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-300) of human ETV6, suggesting that the antibody may recognize full-length ETV6 and possibly N-terminal fragments.
• For western blotting, consider using whole-cell lysates from human, mouse, or rat tissues or cell lines, as cross-reactivity is reported. A band around 53 kDa is expected for the full-length protein.
• ELISA applications may require optimization of coating antigen and detection conditions; recombinant ETV6 protein or specific peptide antigens could serve as positive controls.
• It is advisable to validate the antibody's performance in your specific sample matrix and application, as reactivity may vary depending on endogenous expression levels and post-translational modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutes with a streamlined one-stop sourcing platform for IVD raw materials. Our portfolio includes validated antibody pairs, high-performance monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored to your assay development needs. For targets like ETV6, implicated in hematopoietic disorders and leukemias, we also offer rare target sourcing services—helping you bridge the gap from discovery to diagnostic application efficiently.