Anti-ETV6 Polyclonal Antibody for WB, IHC-P, ELISA - P41212

Core Product Specifications and Parameters

Biological Background: ETV6 Function and Localization

• Transcription factor ETV6 (ETS translocation variant 6), also known as TEL or Tel1, is encoded by the ETV6 gene.
• Functions as a transcriptional repressor by binding to the DNA consensus sequence 5'-CCGGAAGT-3'.
• Plays a critical role in hematopoiesis and malignant transformation; frequently involved in chromosomal rearrangements associated with various leukemias.
• Localizes exclusively to the nucleus where it exerts transcriptional regulatory functions.
• Ubiquitously expressed across tissues.
• Subject to post-translational modifications including acetylation, phosphorylation, and ubiquitin conjugation (Ubl).
• Contains a DNA-binding ETS domain and features an isopeptide bond; identified in proteomics analyses.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment corresponding to amino acids 1-300 of human ETV6; consider this when designing detection strategies or epitope mapping.
• For Western blot (WB), the predicted molecular weight is approximately 53 kDa; validate antibody specificity with appropriate controls in your sample system.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution for paraffin-embedded tissues; ETV6 nuclear localization should yield nuclear staining.
• For ELISA, this antibody may serve in capture or detection formats; consider pairing with a matched antibody pair for quantitative assays.
• Cross-reactivity has been demonstrated with human, mouse, and rat; validate independently for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers with validated antibodies, recombinant proteins, and ancillary reagents from concept to clinic. This anti-ETV6 polyclonal antibody supports research and assay development in hematological malignancies and transcriptional regulation. Explore our comprehensive portfolio of optimized antibodies and bulk raw-material supply solutions to streamline your diagnostic pipeline.