Anti-ETV5 Polyclonal Antibody for WB and ELISA - P41161

Core Product Specifications and Parameters

Biological Background: ETV5 Function and Localization

• ETV5 (ETS translocation variant 5), also known as Ets-related protein ERM, is a member of the ETS transcription factor family.
• It functions as a DNA-binding protein, recognizing the core consensus sequence 5'-GGAA.-3' to regulate gene expression.
• The protein localizes to the nucleus, consistent with its role as a transcription factor.
• It is ubiquitously expressed across various tissues, suggesting broad regulatory functions.
• Alternative splicing generates multiple isoforms of ETV5, contributing to functional diversity.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond and Ubl conjugation), which may modulate its activity and stability.
• The protein's molecular weight is approximately 58 kDa (calculated 57.8 kDa), and it has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 1-510 of human ETV5, covering nearly the full-length protein, which may allow recognition of multiple isoforms and native conformations.
• For Western blotting, the antibody may detect a band around 58 kDa; consider using appropriate cell or tissue lysates expressing ETV5.
• For ELISA, this antibody can be used for quantitative detection of ETV5 protein, but optimization of coating and detection conditions is recommended.
• Due to high sequence homology, the antibody may cross-react with mouse and rat ETV5; validation in the relevant sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this high-quality anti-ETV5 rabbit polyclonal antibody as part of our comprehensive IVD raw material portfolio. In addition to validated antibodies, we supply matched antibody pairs, bulk ancillary reagents, and support rare target sourcing—backed by expert technical and regulatory consultation for diagnostic development.