Anti-ETV4 Polyclonal Antibody for WB and ELISA - P43268

Core Product Specifications and Parameters

Biological Background: ETV4 Function and Localization

• ETV4 (ETS translocation variant 4), also designated PEA3 and E1A-F, is a member of the ETS family of transcription factors, and alternative splice isoforms may be produced.
• It functions as a transcriptional activator, binding to DNA and regulating gene expression in processes such as motor neuron development and keratinocyte differentiation. Related references: PMID:19307308, PMID:31552090
• The protein localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• ETV4 is subject to post-translational modifications including phosphorylation and ubiquitination (isopeptide bond), which may modulate its stability and activity.
• During adenovirus infection, ETV4 binds the viral E1A enhancer and acts as a transcriptional activator with a core binding sequence of 5'-[AC]GGA[AT]GT-3'.
• It is required for the precise positioning of motor neurons within the spinal cord and for controlling terminal arborization of motor neuron axons, partly through regulating cadherin and semaphorin expression.
• Expression of ETV4 has been observed in keratinocytes, where it may contribute to epidermal differentiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 79-178 of human ETV4, an internal region of the protein, which may allow recognition of multiple isoforms if sequence conservation is present.
• For Western blotting, the predicted molecular weight is approximately 54 kDa; consider optimizing blocking and primary antibody dilution for the target species.
• ELISA performance may depend on coating antigen and detection format; titrate the antibody in the relevant sample matrix.
• Cross-reactivity with mouse and rat has been noted, but individual sample validation is recommended when working with these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-ETV4 polyclonal antibody is part of our expanding portfolio for transcription factor and neurogenesis research applications. For reliable sourcing of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare target raw materials, CamelBio is your trusted B2B partner.