Anti-ETV1 Polyclonal Antibody for IHC-P, IF/ICC, ELISA - P50549

Core Product Specifications and Parameters

Biological Background: ETV1 Function and Localization

• ETV1, also known as Ets-related protein 81, is a member of the ETS family of transcription factors.
• Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGA[AT]-3' to regulate gene expression. Related references: PMID:7651741
• Required for olfactory dopaminergic neuron differentiation; may directly activate expression of tyrosine hydroxylase (TH).
• Predominantly localized to the nucleus, consistent with its role in transcription regulation.
• Tissue expression is very high in brain, high in testis, lung, and heart, moderate in spleen, small intestine, pancreas, and colon, and weak or absent in other tissues.
• Subject to phosphorylation and ubiquitination (Ubl conjugation), and characterized as a proto-oncogene involved in chromosomal rearrangements linked to disease variants.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 80-300 of human ETV1, a region that may detect multiple isoforms or uncharacterized variants.
• For IHC-P, include appropriate positive (e.g., brain tissue) and negative controls, and optimize antigen retrieval conditions.
• In IF/ICC, expect nuclear staining; validate with known nuclear markers.
• ELISA applications may require empirical optimization of antibody concentration and pairing.
• As a polyclonal antibody, lot-to-lot consistency should be verified for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a fully integrated supply chain for IVD raw materials, including validated antibodies targeting transcription factors such as ETV1. Our portfolio covers monoclonal/polyclonal antibodies, ancillary reagents, and custom sourcing services to accelerate diagnostic development from concept to clinic. Partner with CamelBio for reliable, scalable access to critical research tools and rare-target raw materials.