Anti-ETV1 Monoclonal Antibody for WB, IP, ELISA, ChIP - P50549

Core Product Specifications and Parameters

Biological Background: ETV1 Function and Localization

• ETV1 (ETS translocation variant 1), also known as ER81, is a member of the ETS family of transcription factors that functions as a transcriptional activator binding to DNA sequences with the consensus 5'-CGGA[AT]-3' (PubMed:7651741). Related references: PMID:7651741
• It is critically required for the differentiation of olfactory dopaminergic neurons, potentially by directly activating the expression of tyrosine hydroxylase (TH) (By similarity).
• ETV1 is predominantly localized to the nucleus and is subject to post-translational modifications including phosphorylation and isopeptide bond formation (ubiquitination).
• The protein exhibits tissue-specific expression, with very high levels in brain, high levels in testis, lung, and heart, moderate expression in spleen, small intestine, pancreas, and colon, and weak or absent expression in placenta and peripheral blood leukocytes.
• ETV1 has been implicated as a proto-oncogene and is involved in chromosomal rearrangements associated with disease variants.
• Alternative splicing of the ETV1 gene generates multiple isoforms, and the protein is identified by proteomics in various contexts.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human ETV1, which may confer specificity for certain isoforms; consider verifying reactivity with the relevant isoform in your experimental system.
• For Western blotting and immunoprecipitation, validate the antibody in lysates from tissues or cell lines with known ETV1 expression (e.g., brain, testis) to confirm the expected band size (~55 kDa).
• When using in ChIP assays, optimize cross-linking and chromatin shearing conditions, as the antibody recognizes a peptide epitope; include appropriate positive and negative control regions.
• ELISA performance may depend on plate coating and detection format; it is advisable to titrate the antibody to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing diagnostic manufacturers and research laboratories with validated antibodies, bulk ancillary reagents, and tailored technical support. This anti-ETV1 antibody supports studies in transcriptional regulation and oncology, and through CamelBio’s supply network, you can access matched antibody pairs, recombinant proteins, and expert guidance to accelerate your diagnostic development workflows.