Anti-ETS2 Polyclonal Antibody for WB, IF/ICC, ELISA - P15036

Core Product Specifications and Parameters

Biological Background: ETS2 Function and Localization

• ETS2 (Protein C-ets-2) is a member of the ETS transcription factor family, encoded by the ETS2 gene.
• It binds specifically to the DNA core motif GGAA/T (Ets-binding site or EBS) and activates transcription of target genes.
• Localizes to the nucleus, consistent with its role in transcriptional regulation.
• Phosphorylation of ETS2 has been reported, which may modulate its transcriptional activity.
• Classified as a proto-oncogene, potentially involved in cell proliferation and tumorigenesis.
• Structural studies (3D-structure keyword) have contributed to understanding its DNA-binding mechanism.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 76-170 of human ETS2, which may confer specific reactivity; validate in the relevant sample system.
• For Western blot (WB), consider using whole-cell lysates from human, mouse, or rat cells to confirm detection of the ~53 kDa band.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to visualize nuclear localization.
• Pair this polyclonal antibody with appropriate secondary detection reagents in ELISA formats, ensuring minimal cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-ETS2 reagent for transcription and cancer research. Our offerings extend to bulk ancillary reagents and support for sourcing rare-target raw materials, bridging the gap from concept to clinic. Let CamelBio streamline your procurement with reliable supply and expert technical support.