Anti-ETS2 Polyclonal Antibody for WB, ELISA - P15036

Core Product Specifications and Parameters

Biological Background: ETS2 Function and Localization

• ETS2 (Protein C-ets-2) is a transcription factor that activates gene expression by binding to DNA.
• It specifically recognizes the GGAA/T core motif (Ets-binding site) in gene promoters.
• The protein contains a conserved ETS DNA-binding domain characteristic of the ETS family.
• ETS2 localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• It is classified as a proto-oncogene, with functions linked to cell proliferation and differentiation.
• ETS2 undergoes phosphorylation, which may regulate its activity and interactions.
• The three-dimensional structure of ETS2 has been resolved, providing insights into its DNA-binding mechanism.
• Predicted molecular weight is approximately 53 kDa (UniProt: 53001 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal peptide within amino acids 300-400 of human ETS2; consider that the epitope may be buried in the full-length protein.
• For western blotting, validate the antibody in the relevant sample system, such as human, mouse, or rat cell lysates, noting the predicted band size of ~53 kDa.
• In ELISA, use optimized peptide coating conditions and confirm specificity with non-target peptides.
• Cross-reactivity with mouse and rat has been reported; verify performance in your experimental context.
• For transcription studies, consider combining this antibody with chromatin immunoprecipitation (ChIP)-grade reagents after empirical validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibodies, optimized reagents, and ancillary supplies. Our product portfolio includes the ETS2 Rabbit pAb, suitable for transcription and oncology research, enabling reliable detection in WB and ELISA applications. As a dedicated partner, we streamline procurement from concept to clinic, offering one-stop access to high-quality raw materials and technical consultation tailored to your diagnostic development needs.