Anti-ETS1 Rabbit Monoclonal Antibody for WB and ELISA - P14921

Core Product Specifications and Parameters

Biological Background: ETS1 Function and Localization

• ETS1, also known as p54, is a member of the ETS transcription factor family and is encoded by the ETS1 gene (also referred to as EWSR2) in humans.
• Directly controls the expression of cytokine and chemokine genes in a wide variety of cellular contexts, and may control the differentiation, survival, and proliferation of lymphoid cells. Related references: PMID:10698492 PMID:11909962 PMID:20378371
• May also regulate angiogenesis through the regulation of gene expression that controls endothelial cell migration and invasion. Related references: PMID:15247905 PMID:15592518
• Acts as a dominant-negative for isoform c-ETS-1A.
• Subcellularly localized to both the nucleus and the cytoplasm.
• Highly expressed within lymphoid cells; isoform expression varies among fetal and adult tissues, and is undetectable in brain and kidney.
• Undergoes various post-translational modifications including acetylation, phosphorylation, and ubiquitination, as evidenced by proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide corresponding to amino acids 1-100 of human ETS1, which suggests recognition of the N-terminal region. Researchers should verify specificity in their own experimental system.
• This antibody is recommended for Western blot (WB) and ELISA according to the supplier. For WB, optimize detection using a suitable dilution of this rabbit monoclonal antibody and an appropriate loading control; the predicted molecular weight is 50 kDa. For ELISA, validate performance with recombinant ETS1 protein or extracts from cells known to express the target.
• Given ETS1’s role as a transcription factor, nuclear extracts may be particularly useful for detection. Consider using appropriate sample preparation protocols to enrich for the protein of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials like this ETS1 monoclonal antibody, supporting studies in transcriptional regulation and immune cell biology. Our one-stop platform offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for reliable assay development. For rare target sourcing needs, our team facilitates custom procurement to accelerate your project timelines.