Anti-ETFDH Rabbit Polyclonal Antibody for WB and ELISA - Q16134

Core Product Specifications and Parameters

Biological Background: ETFDH Function and Localization

• ETFDH encodes the mitochondrial enzyme electron transfer flavoprotein-ubiquinone oxidoreductase, also known as ETF dehydrogenase or ETF:QO.
• It links fatty acid β-oxidation and amino acid catabolism to the respiratory chain by transferring electrons from electron transfer flavoprotein (ETF) to ubiquinone in the mitochondrial inner membrane.
• The protein contains iron-sulfur clusters (4Fe-4S) and FAD, functioning as a key oxidoreductase in lipid degradation and energy metabolism.
• ETFDH is localized to the mitochondrial inner membrane, with an N-terminal transit peptide directing it to the mitochondria.
• Mutations in ETFDH are associated with glutaric aciduria type II (GA II), a disorder of fatty acid and amino acid metabolism.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity and stability.
• Alternative splicing produces multiple transcript variants, contributing to protein diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 318–617) of human ETFDH, which may provide specific recognition of this protein.
• For Western blot, consider a dilution range appropriate for polyclonal antibodies (e.g., 1:500–1:2000) and validate in your sample matrix.
• Due to cross-reactivity with mouse and rat, this antibody may be suitable for studies in rodent models; confirm with appropriate controls.
• ELISA applications can be optimized using direct or indirect detection formats; include non-immune IgG as a negative control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with efficient access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For studies on mitochondrial fatty acid oxidation and metabolic diseases, we source rare-target raw materials like the ETFDH rabbit polyclonal antibody to support your assay development needs. Our one-stop platform streamlines procurement from concept to clinic, ensuring reliable supply for your IVD projects.