Anti-ETFDH Monoclonal Antibody for WB, IF, IHC, ELISA - Q16134

Core Product Specifications and Parameters

Biological Background: ETFDH Function and Localization

• ETFDH (electron transfer flavoprotein-ubiquinone oxidoreductase, also known as ETF dehydrogenase) is a mitochondrial inner membrane flavoprotein that couples fatty acid β-oxidation and amino acid catabolism to the respiratory chain by transferring electrons from electron transfer flavoprotein (ETF) to ubiquinone.
• The enzyme contains FAD and a 4Fe-4S cluster, functioning as an oxidoreductase and participating in electron transport and lipid metabolism.
• Mutations in ETFDH cause glutaric aciduria type II (multiple acyl-CoA dehydrogenase deficiency, MADD), highlighting its role in metabolic disease.
• It localizes to the mitochondrial inner membrane, with a transit peptide directing its mitochondrial import.
• Post-translational modifications include acetylation and phosphorylation, identified through proteomics; alternative splicing generates multiple isoforms.
• The molecular weight predicted from the amino acid sequence is approximately 68.5 kDa, consistent with the observed 68 kDa band on western blots.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 480–617) of human ETFDH, a segment conserved across human, mouse, and rat, supporting the cross-reactivity data.
• For western blotting, a band near 68 kDa is expected; validate detection in relevant tissue or cell lysates and consider optimizing protein loading and blocking conditions.
• When performing immunofluorescence or immunohistochemistry, ensure adequate permeabilization and, for IHC-P, optimize antigen retrieval; validate staining in appropriate positive and negative control samples.
• In ELISA applications, test direct or sandwich formats and titrate the antibody to determine the optimal working concentration for your specific assay.
• Given the mitochondrial localization of ETFDH, subcellular fractionation or mitochondrial enrichment may improve detection sensitivity in certain sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials and technical services to support diagnostic manufacturers and researchers from concept to clinical application. For projects investigating mitochondrial disorders, electron transport chain defects, or metabolic pathways, we provide validated antibody pairs, optimized recombinant antibodies, and bulk ancillary reagents for high-throughput and clinical assay development. Contact our team to discuss custom sourcing for rare-target raw materials and one-stop supply solutions tailored to your workflow.