Anti-ETFA Rabbit Polyclonal Antibody [KO Validated] - P13804

Core Product Specifications and Parameters

Biological Background: ETFA Function and Localization

• ETFA encodes the alpha subunit of the electron transfer flavoprotein (ETF), a mitochondrial heterodimer that accepts electrons from several dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA, and sarcosine dehydrogenase. Related references: PMID:10356313, PMID:15159392, PMID:15975918
• ETF transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Related references: PMID:9334218
• Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. Related references: PMID:12815589, PMID:1430199, PMID:1882842
• The protein contains an FAD cofactor and belongs to the electron transfer flavoprotein family.
• Localized exclusively to the mitochondrial matrix.
• Subject to post-translational modifications including acetylation and phosphorylation; multiple isoforms are produced by alternative splicing.
• Associated with the disease glutaricaciduria type II (multiple acyl-CoA dehydrogenase deficiency, MADD) and plays a key role in lipid degradation and transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human ETFA protein (aa 1-333), suggesting broad epitope coverage typical of polyclonal antibodies.
• Knockout validation (KO Validated) provides confidence in the antibody's specificity; however, optimal working dilutions should be determined empirically for each application.
• For Western blot (WB), the expected band is at approximately 35 kDa. Include a mitochondrial-enriched or purified mitochondrial fraction as a positive control to enhance signal.
• For immunofluorescence (IF/ICC), consider mitochondrial co-staining markers to confirm subcellular localization.
• The antibody is reactive with human, mouse, and rat samples, allowing cross-species studies; verify reactivity in your specific model system if not listed.
• ELISA suitability is indicated; a suitable antigen coating strategy (e.g., recombinant protein or peptide) should be tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs by providing a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies like this anti-ETFA antibody for mitochondrial metabolism studies. Beyond antibodies, we offer one-stop access to matched antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing—streamlining your entire development workflow. For research into fatty acid oxidation disorders and genetic metabolic diseases, our team can also facilitate tailored procurement of related biochemicals and technical support.