Anti-ETFA Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P13804

Core Product Specifications and Parameters

Biological Background: ETFA Function and Localization

• ETFA encodes the alpha subunit of the heterodimeric electron transfer flavoprotein (ETF), which accepts electrons from multiple mitochondrial dehydrogenases, including acyl-CoA, glutaryl-CoA, and sarcosine dehydrogenase
  Related references:
  PMID:10356313, PMID:15159392, PMID:15975918
• ETF subsequently transfers electrons to ETF-ubiquinone oxidoreductase, feeding the main mitochondrial respiratory chain
  Related references:
  PMID:9334218
• Critical for mitochondrial fatty acid β-oxidation and amino acid catabolism; defects in ETFA cause glutaric acidemia type II (GA2), also known as multiple acyl-CoA dehydrogenase deficiency (MADD)
  Related references:
  PMID:12815589, PMID:1430199, PMID:1882842
• The protein localizes to the mitochondrial matrix, consistent with its role in oxidative metabolism.
• Post-translationally modified by acetylation and phosphorylation; also subject to alternative splicing (as per UniProt keywords).
• Belongs to the ETF-alpha family, featuring an FAD-binding domain and a transit peptide for mitochondrial import.

Experimental Guidance and Technical Tips

• For Western blotting, a predominant band around 35 kDa is expected in human, mouse, and rat samples. Due to the recombinant immunogen covering the mature protein, the antibody is likely to recognize native conformations; consider optimizing reducing/non-reducing conditions.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), a mitochondrial staining pattern should be observed. Include appropriate controls (e.g., co-staining with a mitochondrial marker) to confirm specificity.
• For ELISA applications, the antibody may be used as a capture or detection reagent; pairing with a compatible antibody clone is recommended. Validate performance in the intended sample matrix.
• Always titrate the antibody for each new application and sample type to determine the optimal working dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive portfolio of validated IVD raw materials, including optimized monoclonal antibodies like this anti-ETFA clone for metabolic disorder research. Whether you need bulk quantities of validated antibody pairs, ancillary reagents, or assistance sourcing rare targets, our global supply chain and technical consulting can support your assay development from concept to clinic. Let us be your partner in accelerating reliable diagnostic solutions for mitochondrial and metabolic diseases.