Anti-ESYT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BSJ8

Core Product Specifications and Parameters

Biological Background: Extended Synaptotagmin-1 (ESYT1) Function and Localization

• ESYT1 (Extended synaptotagmin-1), also known as Membrane-bound C2 domain-containing protein, is a calcium-binding protein belonging to the extended synaptotagmin family and contains multiple C2 domains and a transmembrane helix.
• It binds calcium via its C2 domains and translocates to endoplasmic reticulum–plasma membrane contact sites in response to elevated cytosolic calcium, where it helps tether the two organelles and promotes membrane appositions. Related references: PMID:23791178 PMID:24183667
• In the absence of cytosolic calcium, ESYT1 acts as an inhibitor of the ADGRD1 G protein-coupled receptor. Related references: PMID:38758649
• It binds glycerophospholipids via a barrel-like domain and may play a role in cellular lipid transport.
• ESYT1 localizes to both the endoplasmic reticulum membrane and the cell membrane.
• The protein is widely expressed in human tissues and undergoes post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 90–220) resides in the N-terminal cytoplasmic region of ESYT1, potentially offering target‑specific reactivity; consider validating specificity in knockout or knockdown models.
• For Western blot, an expected band around 123 kDa corresponds to full‑length ESYT1; include appropriate positive and negative controls from human, mouse, or rat cell lysates.
• In immunofluorescence/ICC, staining may reveal ER and plasma membrane localization; co‑staining with organelle markers is advisable.
• For ELISA, the antibody can be used as a capture or detection reagent; optimize coating concentrations and blocking conditions for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier IVD raw‑material sourcing partner, providing diagnostic developers with validated antibodies, antigen pairs, and ancillary reagents for research and assay development. In the area of ER‑membrane contact site biology, we offer high‑quality ESYT1 polyclonal antibodies suitable for WB, IF, and ELISA, along with technical support to accelerate your projects. Contact us to streamline your procurement needs for rare and established targets.