Anti-ESRRB Rabbit Polyclonal Antibody for WB, ELISA - O95718

Core Product Specifications and Parameters

Biological Background: ESRRB Function and Localization

• ESRRB encodes the steroid hormone receptor ERR2, also known as ERRβ, estrogen-related receptor beta. It is a member of the nuclear receptor subfamily 3 group B.
• Functions as a transcription factor by binding to the canonical ESRRB recognition (ERRE) sequence 5'-TCAAGGTCA-3' on target gene promoters and enhancers, regulating their expression. Related references: PMID:17920186, PMID:19755138
• Plays a critical role in maintaining self-renewal and pluripotency of embryonic and trophoblast stem cells, acting through FGF and Wnt signaling pathways. It is also involved in morula development, particularly at the 8-cell stage.
• Activates key pluripotency genes such as GATA6, NR0B1, POU5F1, and PERM1. Related references: PMID:23836911
• Represses transcriptional activities of NFE2L2 and ESR1, providing regulatory crosstalk. Related references: PMID:17920186, PMID:19755138
• Subcellular localization is primarily nuclear, but it can also be found in the cytoplasm and associated with chromosomes, consistent with its role as a DNA-binding transcription factor.
• Post-translational modifications include acetylation, and it contains zinc-finger motifs essential for DNA binding. Mutations in ESRRB are associated with non-syndromic deafness (DFNB35).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 300–400 of human ESRRB. For Western blot, a band around 48 kDa is expected, although post-translational modifications or isoforms may cause shifts.
• This antibody is suitable for WB and ELISA. For WB, test a range of dilutions (e.g., 1:500–1:2000) and optimize using positive controls such as embryonic stem cell lysates or tissues known to express ESRRB.
• Since cross-reactivity with mouse and rat is indicated, this antibody can be used for studies in these species. Validate specificity in the relevant experimental system, preferably with knockdown or knockout controls.
• For ELISA, consider pairing with a matched detection antibody if used for sandwich assays; the peptide immunogen ensures reactivity with the target protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, CROs, and research laboratories with streamlined access to high-quality IVD raw materials, from antibody pairs and optimized polyclonal/monoclonal antibodies to ancillary reagents. The ESRRB polyclonal antibody supports stem cell and transcriptional regulation research, and CamelBio can also source rare-target reagents and custom production needs. As a single partner covering concept-to-clinic development, CamelBio simplifies procurement while offering technical consultancy to accelerate your project.