Anti-ESRRA Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P11474

Core Product Specifications and Parameters

Biological Background: ESRRA Function and Localization

• ESRRA (estrogen-related receptor alpha) is a steroid hormone receptor and ligand-activated transcription factor belonging to the nuclear receptor subfamily 3 group B (NR3B1). It is also known as ERR1, ERR-alpha, and ESRL1.
• The protein binds to ERR-alpha response elements (ERRE) containing a core consensus half-site 5'-TNAAGGTCA-3', regulating promoters of genes involved in energy metabolism, such as MCAD and lactoferrin.
• ESRRA functions as an obligate dimer and requires interaction with the coactivator PGC-1A (PPARGC1A) for full transcriptional activity; the complex is a key regulator of mitochondrial biogenesis and oxidative metabolism.
• In skeletal muscle, ESRRA induces the expression of PERM1, further linking it to metabolic adaptation and muscle function.
• Subcellular localization includes both nuclear and cytoplasmic compartments, consistent with its roles in transcriptional regulation and potential non-genomic signaling.
• Post-translational modifications such as acetylation and phosphorylation modulate its activity, and the protein features two zinc-finger motifs typical of nuclear receptor DNA-binding domains.
• Alternative splicing generates multiple isoforms, potentially contributing to tissue-specific functions.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–76) of human ESRRA, a domain that may be conserved across isoforms; consider sequence alignment when interpreting isoform detection.
• For WB, a band near 46 kDa is expected; however, post-translational modifications may cause slight shifts. Include appropriate loading controls and molecular weight markers.
• Validate antibody specificity using knockdown or knockout controls, or by overexpressing the target gene in cell lines with low endogenous expression.
• For IF/ICC, note the dual nuclear and cytoplasmic localization; use appropriate fixation and permeabilization protocols to preserve both compartments.
• Cross-reactivity with mouse and rat is indicated; verify performance in the intended species’s tissue or cell lysates with positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B source for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. We can support your ESRRA-related projects in the fields of metabolic disease and nuclear receptor research by providing consistent-quality reagents and rare target sourcing. Our one-stop model streamlines procurement so you can focus on developing robust diagnostic assays.