Anti-ESRRA Rabbit Monoclonal Antibody for WB, ELISA - P11474

Core Product Specifications and Parameters

Biological Background: ESRRA (ERRalpha) Function and Localization

• ESRRA encodes steroid hormone receptor ERR1, also known as estrogen-related receptor alpha (ERRα), a member of the nuclear receptor subfamily 3 group B (NR3B1).
• ERRα functions as a transcription factor that binds to ERRα response elements (ERREs) containing the consensus half-site 5'-TNAAGGTCA-3', regulating target gene expression.
• It is a key regulator of energy metabolism, forming a complex with the coactivator PGC-1α to induce genes such as PERM1 in skeletal muscle and MCAD in mitochondrial fatty acid oxidation.
• ERRα binds to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and the lactoferrin gene promoter, indicating broad transcriptional regulatory roles.
• Subcellularly, ERRα is predominantly nuclear but can also localize to the cytoplasm, consistent with its dynamic roles in transcription and signaling.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation (SUMOylation), which modulate its activity, stability, or subcellular distribution.
• The protein contains zinc finger domains and binds zinc ions, typical of nuclear receptors, and it undergoes alternative splicing, yielding multiple isoforms.

Experimental Guidance and Technical Tips

• The antigen used for immunization is a synthetic peptide from the N-terminal region (amino acids 1-100), which may contribute to targeting a region with lower sequence conservation; verify reactivity in your system if working with highly homologous family members.
• For Western blotting, a band corresponding to ~46 kDa is expected; use appropriate positive controls such as human or mouse tissue lysates known to express ESRRA (e.g., skeletal muscle, heart).
• In ELISA applications, the antibody can be employed as a capture or detection reagent; optimization of coating conditions and antibody concentration is recommended.
• Cross-reactivity with mouse ESRRA is reported, but due to potential sequence differences, validation in mouse samples is advised if precise quantification is required.
• Consider using this antibody in combination with other ERRα antibodies for co-immunoprecipitation or ChIP applications, though these have not been validated by the supplier.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated monoclonal and polyclonal antibodies, antibody pairs, bulk reagents, and rare-target raw material sourcing. Our anti-ESRRA antibody aids research in metabolic disorders and nuclear receptor pathways, and we can support your entire development pipeline with complementary reagents and technical consulting.