Anti-ESRα Rabbit Polyclonal Antibody for WB, ELISA - P03372

Core Product Specifications and Parameters

Biological Background: ESRα (Estrogen Receptor Alpha) Function and Localization

• ESRα, also known as ER-alpha, is a nuclear hormone receptor and a member of the nuclear receptor subfamily 3 group A (NR3A1). It functions as a ligand-activated transcription factor that regulates gene expression in response to estrogen binding.
• Upon estrogen binding, ESRα undergoes conformational changes that promote homodimerization and binding to estrogen response elements (EREs) in target gene promoters, or interaction with other transcription factors such as AP-1, Sp1, and NF-κB, leading to transcriptional activation or repression.
• The receptor plays a critical role in diverse biological processes including cell proliferation, differentiation, and survival in hormone-responsive tissues, and it also mediates rapid membrane-initiated estrogen signaling via kinase cascades.
• Isoforms of ESRα, resulting from alternative promoter usage and splicing, can modulate the transcriptional activity of the full-length receptor through competitive DNA binding or heterodimerization (PubMed:10970861). Related references: PMID:10970861
• ESRα is essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3, and it contributes to maintaining neuronal survival in ischemic reperfusion injury in the presence of estradiol (PubMed:17922032; by similarity). Related references: PMID:17922032
• ESRα also activates NOS3 and endothelial nitric oxide production (PubMed:21937726). Related references: PMID:21937726
• The protein localizes to the nucleus, cytoplasm, cell membrane, and Golgi apparatus, reflecting its involvement in both genomic and non-genomic signaling pathways.
• ESRα is widely expressed in many tissues; however, it is not detected in the pituitary gland (PubMed:10970861). Related references: PMID:10970861

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 516-595) of human ESRα, which is conserved across human and mouse, consistent with the reported cross-reactivity.
• For Western blot, endogenous ESRα runs at approximately 66 kDa; consider using appropriate positive and negative cell/tissue controls (e.g., estrogen receptor-positive cell lines) to verify band specificity.
• For ELISA, the polyclonal antibody can be used as a capture or detection reagent; however, optimal dilutions and conditions should be empirically determined.
• Due to the existence of multiple isoforms, confirm the detected band corresponds to the full-length protein or intended isoform in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For research on ESRα and breast cancer biomarkers, CamelBio offers validated antibody pairs, recombinant proteins, and ancillary reagents to streamline your assay development and procurement workflow.