Anti-ESPN Polyclonal Antibody for WB, IHC-P, ELISA - B1AK53

Core Product Specifications and Parameters

Biological Background: ESPN Function and Localization

• ESPN encodes espin, a multifunctional actin-bundling protein also known as autosomal recessive deafness type 36 protein and ectoplasmic specialization protein.
• Plays a major role in regulating the organization, dimension, dynamics, and signaling capacities of actin filament-rich microvilli in mechanosensory and chemosensory cells (PubMed:29572253). Related references: PMID:29572253
• Required for the assembly and stabilization of stereociliary parallel actin bundles and crucial for the formation and maintenance of inner ear hair cell stereocilia.
• Involved in the elongation of actin in stereocilia and in targeting MYO3B to stereocilia tips in extrastriolar hair cells, regulating stereocilia diameter and staircase formation.
• Localizes to the cytoplasm, cytoskeleton, and cell projections including stereocilia and microvilli.
• Contains ANK repeats and a coiled-coil domain; undergoes alternative splicing and phosphorylation.
• Associated with non-syndromic deafness DFNB36, Usher syndrome type I (USH1M), and retinitis pigmentosa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 585–854), so the antibody may recognize full-length espin and isoforms containing this segment. Verify reactivity in your target tissue or cell line.
• For Western blotting, espin has a predicted molecular weight of approximately 92 kDa; consider using appropriate positive controls such as inner ear tissue or ESPN-expressing cells.
• When performing IHC on paraffin-embedded sections (IHC-P), optimize antigen retrieval conditions and antibody dilution for your sample type.
• ELISA can be employed for detecting espin protein levels or for antibody titering.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner for diagnostic manufacturers and research laboratories, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-ESPN antibody supports investigations into hearing loss, stereocilia biology, and the actin cytoskeleton. Leverage CamelBio's sourcing expertise to efficiently obtain rare-target raw materials and accelerate your project from concept to clinic.