Anti-ESPL1 Rabbit Polyclonal Antibody for WB, ELISA - Q14674

Core Product Specifications and Parameters

Biological Background: ESPL1 (Separin) Function and Localization

• The ESPL1 gene encodes separin, also known as separase, a caspase-like cysteine protease essential for chromosome segregation during mitosis.
• Separin cleaves the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase, triggering sister chromatid separation.
• During most of the cell cycle, separase activity is tightly regulated through multiple inhibitory mechanisms to prevent premature cohesin cleavage.
• The protein contains autocatalytic cleavage sites and undergoes phosphorylation, as reflected in its annotation as a phosphoprotein.
• Subcellular localization includes both the cytoplasm and nucleus, consistent with its dynamic role in mitotic regulation.
• Alternative splicing of ESPL1 generates multiple isoforms, expanding functional diversity.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein corresponding to amino acids 1220-1500 of human ESPL1; consider this immunogen region when designing complementary detection reagents.
• Suitable applications include western blot (WB) and ELISA, as indicated by the supplier; optimal dilutions should be determined empirically for your experimental system.
• Cross-reactivity is reported with human, mouse, and rat samples; validate performance in the relevant species and sample type before critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials. This ESPL1 (separase) antibody supports cell cycle and chromosome biology research, and CamelBio can assist with sourcing matched antibody pairs, ancillary reagents, and bulk quantities to accelerate your assay development. Explore our portfolio for rare-target reagents and customized procurement solutions.