Anti-ESE1/ELF3 Rabbit Monoclonal Antibody for WB, ELISA, ChIP, CUT&Tag - P78545

Core Product Specifications and Parameters

Biological Background: ESE1/ELF3 Function and Localization

• ESE1/ELF3 (ETS-related transcription factor Elf-3), also known as ERT, ESX, EPR-1, ESE-1, or E74-like factor 3, is a member of the ETS transcription factor family. It is encoded by the ELF3 gene and generates multiple isoforms through alternative splicing.
• It functions as a transcriptional activator that binds to ETS sequences containing the consensus core GGA[AT] and transactivates promoters such as collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2, and TGM3.
• ELF3 acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter, while repressing the KRT4 promoter, highlighting dual regulatory roles.
• The protein is involved in mediating vascular inflammation and serves as a critical downstream effector of the ERBB2 signaling pathway, thereby playing important roles in epithelial cell differentiation and tumorigenesis.
• ELF3 may be associated with mammary gland development and involution, and is essential for transcription with TATA-less promoters in preimplantation embryos, which is crucial for preimplantation development (By similarity).
• It localizes to both the cytoplasm and nucleus, as indicated by subcellular location data.
• Tissue expression is restricted to epithelial cell-rich tissues such as mammary gland, colon, trachea, kidney, prostate, uterus, stomach, and skin.
• Additional keywords reflect its involvement in developmental processes, differentiation, DNA binding, inflammatory response, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 371 amino acids of human ESE1/ELF3; thus, the antibody may detect full-length ELF3 and certain isoforms. Validate reactivity in your sample system.
• In Western blot, a dominant band near 41 kDa is expected; however, alternative splicing or post-translational modifications may produce additional bands. Optimize denaturing and reducing conditions for your lysates.
• The antibody is suitable for ELISA-based detection; appropriate titration and control proteins are recommended to ensure assay linearity and specificity.
• For ChIP and CUT&Tag experiments, confirm compatibility with your chromatin preparation protocol. Because ELF3 is a DNA-binding transcription factor, cross-linking and sonication conditions may impact epitope availability.

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