Anti-ESAM Rabbit Polyclonal Antibody for WB, ELISA - Q96AP7

Core Product Specifications and Parameters

Biological Background: ESAM Function and Localization

• ESAM (endothelial cell-selective adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin superfamily, featuring an extracellular immunoglobulin domain.
• It mediates cell-cell adhesion primarily through homophilic molecular interactions, critical for maintaining endothelial barrier integrity.
• ESAM localizes to cell junctions, including adherens junctions and tight junctions, as well as the cell membrane.
• Highly expressed in endothelial cells, underscoring its specialized role in vascular biology.
• Post-translational modifications include disulfide bond formation, glycosylation, and phosphorylation, which may regulate its function.
• Alternative splicing generates multiple isoforms, potentially expanding its functional repertoire.
• The protein is associated with disease variants and intellectual disability, hinting at broader physiological roles.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 30-248), suggesting reactivity with both native and denatured ESAM; consider this when designing assays.
• For Western blot applications, reducing conditions are typically compatible; validate detection using endothelial cell lysates (e.g., HUVEC) as a positive control.
• In ELISA setups, the antibody may be suited as a capture or detection reagent; performance should be verified with recombinant ESAM protein or cell-based systems.
• Due to high expression in endothelial cells, tissue sample choice is critical; avoid tissues with low ESAM levels to ensure signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and customized monoclonal/polyclonal antibody development. Supporting ESAM-focused research and diagnostic development, we supply high-quality polyclonal antibodies and related reagents to accelerate your vascular and cell-adhesion studies. From concept to clinic, our streamlined procurement ensures dependable supply for your assay needs.