Anti-ERp57 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P30101

Core Product Specifications and Parameters

Biological Background: ERp57 Function and Localization

• Protein disulfide-isomerase A3 (ERp57, GRP58) catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone. Related references: PMID:11825568 PMID:16193070 PMID:27897272
• Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it acts as an essential folding chaperone for TAPBP, assisting the dynamic assembly of MHC I with high-affinity antigens. Related references: PMID:35948544 PMID:36104323
• Plays a crucial role in the presentation of antigens to cytotoxic T cells, thereby contributing to adaptive immunity.
• Subcellular localization includes the endoplasmic reticulum lumen and melanosomes.
• Detected in the flagellum and head region of spermatozoa, and expressed in liver, stomach, and colon, notably in gastric parietal cells and chief cells.
• Features a redox-active center, disulfide bonds, and undergoes post‑translational modifications such as acetylation, phosphorylation, and methylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 100–200 of human ERp57 (P30101). Verify reactivity in your specific sample system and consider epitope accessibility under native vs. denaturing conditions.
• For Western blotting, the predicted molecular weight is 57 kDa. Include appropriate positive and negative controls (e.g., human cell lines known to express ERp57).
• In immunohistochemistry (paraffin), validate staining in tissues with established ERp57 expression (liver, stomach, colon) and optimize antigen retrieval.
• For ELISA, perform standard dilution optimization and consider pairing with detection antibodies for sandwich formats.

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