Anti-ERp57 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P30101

Core Product Specifications and Parameters

Biological Background: ERp57 Function and Localization

• ERp57 (also designated PDIA3, GRP58, ERp60, or ERp61) is a member of the protein disulfide isomerase family and functions as an oxidoreductase and molecular chaperone in the endoplasmic reticulum.
• It catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in substrate proteins, facilitating correct protein folding. Related references: PMID:11825568, PMID:16193070, PMID:27897272
• ERp57 is a core component of the major histocompatibility complex class I (MHC I) peptide loading complex, where it interacts with TAPBP to assist the assembly of stable MHC I–peptide complexes, playing a critical role in adaptive immunity. Related references: PMID:35948544, PMID:36104323
• Subcellularly, the protein resides primarily in the endoplasmic reticulum lumen and has also been identified in melanosomes.
• At the tissue level, ERp57 is expressed in liver, stomach, and colon, and is detected in gastric parietal cells, chief cells, and the flagellum and head region of spermatozoa. Related references: PMID:20400973, PMID:24188822
• Post‑translational modifications include acetylation, methylation, and phosphorylation; the protein also contains a redox‑active center and disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 206–505 of human ERp57, covering the C‑terminal region that includes the two catalytic thioredoxin‑like domains; this design may allow recognition of both oxidized and reduced enzyme forms.
• For western blotting, a band migrating around 54 kDa is expected under reducing conditions; a reducing sample buffer and 4–20% gradient gel can be used for improved resolution.
• In immunohistochemical (IHC‑P) and immunofluorescence (IF/ICC) applications, optimise antigen retrieval and permeabilization conditions as ERp57 is an ER‑lumenal protein; consider validation in the relevant sample system.
• For ELISA, the antibody can be employed for detection of ERp57 in cell lysates or recombinant protein preparations; perform cross‑validation with other methods to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies like this anti‑ERp57 pAb, for immunoassay development. Our one‑stop sourcing model also provides access to optimized recombinant proteins, ancillary reagents, and rare‑target raw‑material procurement services. Whether you need bulk antibodies, custom assay development, or technical consultation, CamelBio accelerates your project from concept to clinical application.