Anti-ERP44 Rabbit Polyclonal Antibody for WB, ELISA - Q9BS26

Core Product Specifications and Parameters

Biological Background: ERP44 Function and Localization

• Also known as PDIA10 and TXNDC4, ERP44 is a member of the protein disulfide isomerase family characterized by a thioredoxin domain.
• Core function: mediates thiol-dependent retention of substrates in the early secretory pathway by forming transient mixed disulfides via the conserved CRFS motif.
  Related references: PMID:11847130 PMID:14517240
• Inhibits the calcium channel activity of ITPR1, modulating intracellular calcium signaling.
  Related references: PMID:15652484
• Required for retaining ERO1A and ERO1B in the endoplasmic reticulum, thus regulating oxidative protein folding.
  Related references: PMID:11847130 PMID:29858230
• Localizes to the endoplasmic reticulum lumen and participates in ER stress response and chaperone activity.
• Keywords: Chaperone, Stress response, Disulfide bond, Endoplasmic reticulum.
• Has a molecular weight of approximately 47 kDa, consistent with the UniProt-calculated mass of 46.97 kDa.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 57-406) covers a large portion of the protein, making the antibody likely to recognize native ERP44. For WB, a band around 47 kDa is expected; consider using appropriate controls and molecular weight markers.
• Validated for WB and ELISA; for ELISA, consider coating the antigen or antibody accordingly and optimize detection conditions.
• Since cross-reactivity is reported for human, mouse, and rat, this antibody can be used for comparative studies across these species. Validate in the relevant sample system as expression levels may vary.
• For functional studies, note that ERP44 forms transient disulfide bonds; avoid reducing agents if native complex detection is needed, but standard reducing WB is suitable for total protein detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibodies like this Anti-ERP44 Polyclonal Antibody, to support research in endoplasmic reticulum biology and protein folding disorders. With capabilities in bulk supply, custom immunogen design, and ancillary reagent sourcing, CamelBio streamlines the transition from concept to clinic.