Anti-ERp29 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P30040

Core Product Specifications and Parameters

Biological Background: ERp29 Function and Localization

• ERp29, also known as ERp28, ERp31, PDIA9, and PDI-DB, is an endoplasmic reticulum (ER)-resident protein encoded by the ERP29 gene (C12orf8) in humans.
• It does not exhibit disulfide isomerase activity but instead plays a critical role in the processing and folding of secretory proteins within the ER lumen.
• ERp29 localizes primarily to the ER lumen and has also been detected in melanosomes, indicating involvement in secretory and pigmentation pathways.
• The protein is ubiquitously expressed, with higher levels in secretory tissues, aligning with its proposed function in protein secretion.
• Post-translational modifications include phosphorylation, and the gene undergoes alternative splicing, generating multiple transcript variants.
• Structural studies (3D-structure keyword in UniProt) have resolved its domain organization, aiding in understanding its functional interactions.
• The predicted molecular weight is approximately 29 kDa (UniProt: 28993 Da), matching the observed protein weight in experimental settings.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 33–261, covering the mature protein after signal peptide removal; it is expected to recognize both native and denatured ERp29 in applications like WB and IF/ICC.
• For Western blot (WB), a band near 29 kDa is anticipated; use lysis buffers optimized for ER luminal proteins and include protease/phosphatase inhibitors.
• In immunohistochemistry (IHC-P), antigen retrieval (e.g., heat-induced citrate buffer) may improve staining in FFPE tissues; validate using secretory tissues with known high ERp29 expression.
• For immunofluorescence (IF/ICC), permeabilization is essential to access the ER lumen; co-staining with ER markers (e.g., calnexin) can confirm localization specificity.
• ELISA-based assays can be optimized using the recombinant immunogen as a reference standard; appropriate blocking and washing steps should be empirically determined.
• Reported cross-reactivity with mouse and rat ERp29 (84% and 83% sequence identity, respectively) supports cross-species studies; however, validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research laboratories. This anti-ERp29 antibody supports investigations into ER protein folding and secretory pathway regulation. As a one-stop sourcing platform, we provide validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and customized rare-target procurement to accelerate your projects from concept to clinic.