Anti-ERp29 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P30040

Core Product Specifications and Parameters

Biological Background: ERp29 Function and Localization

• ERp29 (Endoplasmic reticulum resident protein 29), also known as ERp28 and ERp31, is encoded by the ERP29 gene (also known as C12orf8, ERP28). It is a member of the protein disulfide isomerase (PDI) family but lacks disulfide isomerase activity.
• ERp29 plays a critical role in the processing of secretory proteins within the endoplasmic reticulum (ER), likely by facilitating protein folding and quality control in the ER lumen.
• The protein is ubiquitously expressed, with higher levels in secretory tissues, consistent with its role in ER protein processing.
• Subcellular localization is primarily the endoplasmic reticulum lumen, and it has also been detected in melanosomes, suggesting potential roles in specialized secretory organelles.
• Keywords include phosphoprotein (indicating regulation by phosphorylation) and alternative splicing, suggesting the existence of multiple isoforms, as supported by its molecular weight of approximately 29 kDa.
• ERp29 does not function as a classical disulfide isomerase, distinguishing it from other PDI family members, and may instead act as a molecular chaperone or folding assistant.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-102) of human ERp29; this region is conserved in mouse and rat, consistent with the reported cross-reactivity. For applications, validate the antibody's specificity in your target species using appropriate positive and negative controls.
• For Western blot, ERp29 migrates at approximately 29 kDa; ensure proper protein loading and consider using reducing conditions. Due to potential post-translational modifications, minor band shifts may occur.
• In immunohistochemistry (IHC-P), antigen retrieval methods may be required; test standard protocols with recommended detection systems. The ubiquitous but secretory tissue enrichment may inform tissue selection for IHC.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with compatible secondary antibodies is essential. Validate performance against recombinant ERp29 protein or cell lysates.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material supplier, CamelBio provides diagnostic manufacturers and laboratories with reliable access to key reagents like the anti-ERp29 rabbit monoclonal antibody for ER biology and secretory pathway studies. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. Partner with CamelBio to streamline your diagnostic and research workflows from concept to clinic.