Anti-ERp19 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - O95881

Core Product Specifications and Parameters

Biological Background: Thioredoxin Domain-Containing Protein 12 Function and Localization

• Thioredoxin domain-containing protein 12 (TXNDC12) is also known as endoplasmic reticulum resident protein 19 (ERp19), ER protein 18 (ERp18), and thioredoxin-like protein p19 (hTLP19). It is encoded by the TXNDC12 gene (also designated TLP19).
• It functions as a protein-disulfide reductase in the endoplasmic reticulum, promoting disulfide bond formation in client proteins through its thiol-disulfide oxidase activity.
• TXNDC12 contains a thioredoxin domain, characteristic of oxidoreductases, and features a redox-active center with a CXXC motif.
• The protein is localized to the endoplasmic reticulum lumen, where it participates in protein folding and quality control.
• ERp19 is widely expressed across tissues, consistent with its fundamental role in ER protein processing.
• Post-translational modifications include a signal peptide for ER targeting, and the protein forms disulfide bonds as part of its catalytic cycle.
• The protein has been studied by 3D structure determination, direct protein sequencing, and proteomics identification, underscoring its well-characterized biochemistry.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide spanning amino acids 1-100 of human ERp19. This region may confer specificity for ERp19 versus other thioredoxin proteins.
• For western blot (WB), a band around 19 kDa (calculated molecular weight ~19.2 kDa) may be observed; however, post-translational modifications or sample conditions could shift migration.
• In immunohistochemistry (IHC-P), the antibody may detect ERp19 in paraffin-embedded tissue sections, but validation in the relevant sample system is recommended.
• ELISA applications can be considered, though optimization of coating and detection conditions is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including antibodies and reagents for studying endoplasmic reticulum biology and protein folding. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development. For rare or emerging targets like ER oxidoreductases, our expert sourcing team can help accelerate your project from concept to clinic.