Anti-Erlin-2 Monoclonal Antibody for WB, IHC-P, ELISA - O94905

Core Product Specifications and Parameters

Biological Background: Erlin-2 Function and Localization

• Erlin-2 (ERLIN2), also known as SPFH2, NET32, or SPG18, is a 38 kDa endoplasmic reticulum (ER) membrane protein belonging to the stomatin-prohibitin-flotillin-HflC/K (SPFH) domain superfamily.
• It forms a complex with Erlin-1 and mediates ER-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs), regulating calcium signaling. Related references: PMID:17502376, PMID:19240031
• Erlin-2 promotes sterol-accelerated ERAD of HMG-CoA reductase (HMGCR), a key enzyme in cholesterol biosynthesis, likely via the AMFR/gp78 ubiquitin ligase complex. Related references: PMID:21343306
• It participates in cholesterol homeostasis by regulating SREBP signaling, potentially retaining the SCAP-SREBF complex in the ER. Related references: PMID:24217618
• The protein localizes to the ER membrane and is ubiquitously expressed across human tissues.
• Keywords from UniProt highlight its involvement in cholesterol and lipid metabolism, neurodegeneration (mutations linked to hereditary spastic paraplegia SPG18), and post-translational modifications including acetylation and glycosylation.
• Alternative splicing yields multiple isoforms, and the protein undergoes direct sequencing and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 50–150 of human Erlin-2, which should theoretically recognize all known isoforms and cross-react with mouse and rat orthologs.
• For Western blot (WB), a band at ~38 kDa is expected. Use appropriate positive controls such as HeLa, HEK293, or liver lysates.
• For immunohistochemistry (IHC-P), consider optimizing antigen retrieval (e.g., citrate buffer pH 6.0) and validate in tissues with known Erlin-2 expression.
• ELISA applications are possible for screening or titering; ensure proper coating conditions and detection range.
• As a rabbit monoclonal antibody, it offers batch-to-batch consistency and high specificity; however, always confirm performance in your target sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-Erlin-2 monoclonal antibody serves as a reliable tool for studying endoplasmic reticulum-associated degradation and cholesterol metabolism, facilitating biomarker discovery and assay development. Beyond individual products, CamelBio specializes in rare target sourcing and custom solutions, helping you accelerate every stage from concept to clinic.