Anti-ERK2 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P28482

Core Product Specifications and Parameters

Biological Background: ERK2 Function and Localization

• ERK2 (MAPK1, p42-MAPK) is a serine/threonine kinase and a core component of the MAPK/ERK cascade, essential for mediating diverse cellular responses such as growth, adhesion, survival, and differentiation through regulation of transcription, translation, and cytoskeletal rearrangements.
• As a central signaling kinase, ERK2 phosphorylates over 160 substrates localized across the nucleus, cytosol, and organelles, including transcription factors (e.g., ATF2, FOS, ELK1), apoptosis regulators (e.g., BAD, MCL1), and translational regulators (e.g., EIF4EBP1). Related references: PMID:35831023
• Under hypoxic conditions, ERK2 phosphorylates phosphoglycerate kinase PGK1 at the mitochondria, suppressing acetyl-coenzyme A formation from pyruvate. Related references: PMID:26942675
• ERK2 promotes smoothened signaling by phosphorylating GLI1, leading to its dissociation from SUFU and nuclear translocation. Related references: PMID:35831023
• The protein dynamically localizes to multiple subcellular compartments including the cytoplasm, nucleus, mitotic spindle, centrosome, caveola, and focal adhesions, reflecting its pleiotropic functions.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination regulate its activity, subcellular distribution, and interactions, while additional keywords highlight its roles in apoptosis, cell cycle, host-virus interactions, and transcriptional repression.
• An alternative function independent of kinase activity acts as a transcriptional repressor by binding to specific DNA sequences to downregulate interferon-gamma-induced genes.

Experimental Guidance and Technical Tips

• The immunogen lies within amino acids 200–300, a conserved region shared with ERK1; consider verifying specificity with knockout controls or isoform-specific blocking peptides.
• This rabbit monoclonal antibody is suitable for WB, IHC-P, and ELISA applications; optimize dilutions and antigen retrieval conditions for your specific sample type.
• Cross-reactivity with mouse and rat ERK2 allows for translational studies; validate performance in the relevant species’ tissue or cell lysates before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your diagnostic development. This ERK2 antibody empowers research into MAPK/ERK signaling pathways central to oncology, neuroscience, and inflammatory diseases. From single-antibody sourcing to rare-target raw-material procurement, CamelBio bridges your concept-to-clinic journey with comprehensive supply capabilities.