Anti-ERK1/ERK2 Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P28482 / P27361

Core Product Specifications and Parameters

Biological Background: ERK1/ERK2 Function and Localization

• MAPK1 (ERK2, p42-MAPK) and MAPK3 (ERK1, p44-MAPK) are closely related serine/threonine kinases essential for the canonical MAPK/ERK signal transduction cascade.
• They phosphorylate a broad array of substrates (over 160) including transcription factors (ATF2, ELK1, FOS), cytoskeletal regulators, and other protein kinases, mediating responses in cell proliferation, differentiation, adhesion, and apoptosis. Related references: PMID:35831023
• In hypoxic microenvironments, ERK2 directly phosphorylates phosphoglycerate kinase PGK1 at the mitochondrion, altering central carbon metabolism. Related references: PMID:26942675
• The kinase also participates in spindle assembly checkpoint control and Golgi fragmentation during mitosis, highlighting roles in cell cycle progression.
• Subcellularly, ERK2 localizes dynamically to the cytoplasm, nucleus, centrosome, focal adhesions, and caveolae, reflecting its context-dependent signaling functions.
• Post-translational modifications, including phosphorylation, acetylation, and ubiquitination, tightly regulate ERK2 activity and stability.
• Alternative splicing generates distinct isoforms, and mutations in MAPK1 are linked to human diseases, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 200–300 of human ERK1/ERK2 (NP_620407.1/NP_002737.2), a conserved region between the two isoforms, making this antibody suitable for detecting both ERK1 and ERK2.
• For Western blotting (WB), expect bands around 42–44 kDa for ERK1 and 41–42 kDa for ERK2; additional lower bands may represent phosphorylated forms or degradation products.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), validate antibody specificity using appropriate controls (e.g., siRNA knockdown or peptide blocking) due to broad expression.
• The antibody cross-reacts with mouse and rat, but always confirm reactivity in your specific experimental model.
• ELISA applications may require optimization of coating antigen and detection conditions; the antibody is suitable as a capture or detection reagent in sandwich formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, connecting diagnostic developers with high-quality antibodies like this anti-ERK1/ERK2 rabbit polyclonal antibody. Whether you need validated antibody pairs for ELISA development, bulk monoclonal/polyclonal antibodies, or rare-target sourcing, our one-stop platform supports your project from concept to clinic. With ERK1/ERK2 playing pivotal roles in cancer and cell signaling research, we also provide ancillary reagents and technical consulting to accelerate your assay development.