Anti-ERK1/2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P28482/P27361

Core Product Specifications and Parameters

Biological Background: ERK1/2 Function and Localization

• ERK1 (MAPK3, P27361) and ERK2 (MAPK1, P28482) are the two main mitogen-activated protein kinases, central to the MAPK/ERK signaling cascade that regulates cell proliferation, differentiation, survival, and apoptosis.
• As serine/threonine kinases, they phosphorylate a vast array of substrates (>160 identified) upon activation, including transcription factors (e.g., ATF2, ELK1, FOS), cytoskeletal proteins, apoptosis regulators, translation regulators, and other kinases and phosphatases, thereby propagating and amplifying the signal. Related references: PMID:35831023
• Under hypoxic conditions, ERK2 phosphorylates phosphoglycerate kinase PGK1 at the mitochondrial outer membrane, promoting its mitochondrial translocation and suppressing the conversion of pyruvate to acetyl-coenzyme A, highlighting a role in metabolic reprogramming. Related references: PMID:26942675
• In the cytoplasm, ERK2 positively regulates smoothened signaling by phosphorylating GLI1, which promotes GLI1 dissociation from SUFU and its subsequent translocation to the nucleus, linking ERK signaling to developmental pathways. Related references: PMID:35831023
• ERK2 also functions as a transcriptional repressor, binding directly to DNA and repressing interferon gamma-induced genes, independent of its kinase activity.
• Subcellularly, ERK1/2 are dynamically distributed: they localize to the cytoplasm, nucleus, mitotic spindle, centrosome, focal adhesions, and caveolae, reflecting their diverse roles in signal transduction, cell cycle, and cytoskeletal regulation.
• Keywords associated with ERK1/2 include ATP-binding, nucleotide-binding, serine/threonine-protein kinase, phosphoprotein (acetylation and ubiquitination), and involvement in host-virus interactions, emphasizing their regulation and broad cellular impact.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein corresponding to amino acids 180-360 of human ERK2; therefore, the antibody may recognize both ERK1 and ERK2 due to high sequence homology in this region.
• In Western blotting, expect bands at approximately 42 kDa (ERK2) and 44 kDa (ERK1) in lysates from human, mouse, and rat cells. Use validated positive controls such as HeLa, HEK293, or NIH/3T3 whole-cell lysates.
• For immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions, as ERK1/2 subcellular localization can change upon stimulation.
• The antibody has been tested for ELISA; consider using it for quantitative assays or phosphorylation studies after appropriate validation.
• Cross-reactivity with other species beyond human, mouse, and rat has not been tested; perform a species-specific validation if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic and research workflows. This anti-ERK1/2 antibody, targeting the critical MAPK pathway, exemplifies our commitment to providing high-quality reagents for cell signaling studies and assay development. Let CamelBio streamline your supply chain from concept to clinic.